Immunology Practice Questions (ASCP MLS and MLT)
5–10% of the MLT exam · 5–10% of the MLS exam, in ASCP’s content guidelines
100 questions across all seven content areas in 2 hours 30 minutes, with a score for Immunology so you know where you stand.
Free Immunology drillWhich result shows past rubella infection or vaccination with immunity?
Immunology on the ASCP exams
Immunology is 5–10% of both the MLT and MLS exams. Questions cover the immune response, immunoglobulins and complement, diseases of the immune system, transplantation and HLA, infectious disease serology and how immunoassays work.
- Share of the exam
- MLT 5–10% (7 of 100 on our MLT mock) · MLS 5–10% (7 of 100 on our MLS mock)
- Our drill
- 10 questions from 38 immunology questions, untimed, an explanation after every answer; one free attempt, unlimited with a pass
What Immunology questions test
- Immune system basics: B and T cells, MHC, immunoglobulin classes and structure, complement pathways, prozone and titers
- Diseases: autoimmunity and ANA patterns, the four hypersensitivity types, monoclonal gammopathies and immunodeficiencies
- Transplantation: HLA inheritance, graft-versus-host disease and tumor markers
- Serology: hepatitis B patterns, HCV and HIV testing algorithms, EBV, rubella and syphilis testing (treponemal and nontreponemal)
- Procedures: ELISA, competitive and sandwich immunoassays, immunofluorescence and agglutination
Sit the full exam under the real clock
100 questions across all seven content areas in 2 hours 30 minutes, with a score for Immunology so you know where you stand.
Free Immunology drillHow to study it
- Learn the hepatitis B serologic patterns as a table.
- Know the current CDC HIV algorithm: antigen/antibody screen, differentiation assay, then RNA.
- Pair each autoantibody with the disease it is most specific for.
Common questions
How many immunology questions are on the ASCP exam?
Immunology is 5–10% of both the MLT and MLS exams. Our mocks include 7 immunology questions each.
Does immunology include blood bank immunology?
Blood group immunology is part of the Blood Banking area. The Immunology area covers immune system principles, immune disease, transplantation and infectious disease serology.
100 questions across all seven content areas in 2 hours 30 minutes, with a score for Immunology so you know where you stand.
Free Immunology drillThe other content areas
Sources
- ASCP BOC: MLT and MLS Examination Content Guidelines (revised June 9, 2026)
- ASCP BOC: About the Examination (format, CAT, scoring, field test questions, calculator)
- ASCP BOC: Medical Laboratory Technician, MLT(ASCP): eligibility routes and $235 fee
- ASCP BOC: Medical Laboratory Scientist, MLS(ASCP): eligibility routes and $260 fee
- ASCP BOC: Examination Results
- ASCP BOC: After the Examination (re-applying, five attempts per route, 30-day wait)
- ASCP BOC: Schedule or Reschedule Your Examination (Pearson testing centers)
- ASCP BOC: MLS and MLT Credential Maintenance Program requirements (36 points, three years)
Original practice questions for exam preparation, written to the ASCP BOC MLT and MLS Examination Content Guidelines (revised June 9, 2026), not ASCP exam questions. Independent practice, not affiliated with or endorsed by the American Society for Clinical Pathology (ASCP), the ASCP Board of Certification or Pearson VUE.