Blood Bank Practice Questions (ASCP MLS and MLT)
15–20% of the MLT exam · 17–22% of the MLS exam, in ASCP’s content guidelines
100 questions across all seven content areas in 2 hours 30 minutes, with a score for Blood Banking so you know where you stand.
Free Blood Banking drillWhich laboratory finding is part of the initial investigation of a suspected hemolytic transfusion reaction?
Blood Banking on the ASCP exams
Blood Banking (immunohematology) is 15–20% of the MLT exam and 17–22% of the MLS exam. Questions cover how components are prepared, stored and selected, how blood group antigens are inherited and detected, how antibodies are identified, and how to recognize and work up transfusion reactions.
- Share of the exam
- MLT 15–20% (17 of 100 on our MLT mock) · MLS 17–22% (20 of 100 on our MLS mock)
- Our drill
- 10 questions from 76 blood banking questions, untimed, an explanation after every answer; one free attempt, unlimited with a pass
What Blood Banking questions test
- Components: storage temperatures, anticoagulant and additive shelf lives, expiration after irradiation, washing, pooling and thawing, and quality control limits
- Donors: FDA eligibility criteria such as hemoglobin, temperature and donation intervals, donor reactions and required donor testing
- Blood group systems: ABO and H biochemistry, subgroups and discrepancies, Rh notation and weak D, Lewis, Kell, Kidd, Duffy, MNS and P antigens, and which are destroyed by enzymes or DTT
- Testing: antibody screen and panel interpretation, dosage, the rule of three, DAT and check cells, elutions, adsorptions and neutralization
- HDFN and RhIG: antenatal and postpartum dosing, rosette and Kleihauer-Betke testing, and the RhIG vial calculation
- Transfusion practice: ABO compatibility of red cells and plasma, acute and delayed hemolytic reactions, TRALI, TACO, septic and allergic reactions
Sit the full exam under the real clock
100 questions across all seven content areas in 2 hours 30 minutes, with a score for Blood Banking so you know where you stand.
Free Blood Banking drillHow to study it
- Memorize the storage and expiration table first; it supplies many quick questions.
- Work panels by crossing out antigens on nonreactive cells, then check dosage and the rule of three.
- Learn which antibodies are IgM (cold, saline) and which are IgG (warm, AHG), and which enzymes destroy which antigens.
- Practice the RhIG calculation until you can do it in under a minute.
Common questions
How much of the ASCP exam is blood bank?
Blood Banking is 15–20% of the MLT exam and 17–22% of the MLS exam under the content guidelines revised June 9, 2026. Our MLT mock includes 17 blood bank questions and our MLS mock 20.
Which donor eligibility rules does the exam use?
ASCP says its blood banking donor eligibility questions are based on regulations current as of June 2026, and its regulatory questions are based on US sources such as AABB, FDA and CLIA.
100 questions across all seven content areas in 2 hours 30 minutes, with a score for Blood Banking so you know where you stand.
Free Blood Banking drillThe other content areas
Sources
- ASCP BOC: MLT and MLS Examination Content Guidelines (revised June 9, 2026)
- ASCP BOC: About the Examination (format, CAT, scoring, field test questions, calculator)
- ASCP BOC: Medical Laboratory Technician, MLT(ASCP): eligibility routes and $235 fee
- ASCP BOC: Medical Laboratory Scientist, MLS(ASCP): eligibility routes and $260 fee
- ASCP BOC: Examination Results
- ASCP BOC: After the Examination (re-applying, five attempts per route, 30-day wait)
- ASCP BOC: Schedule or Reschedule Your Examination (Pearson testing centers)
- ASCP BOC: MLS and MLT Credential Maintenance Program requirements (36 points, three years)
Original practice questions for exam preparation, written to the ASCP BOC MLT and MLS Examination Content Guidelines (revised June 9, 2026), not ASCP exam questions. Independent practice, not affiliated with or endorsed by the American Society for Clinical Pathology (ASCP), the ASCP Board of Certification or Pearson VUE.